Jingyi Jessica Li, ClusterDE: a post-clustering differential expression (DE) method robust to false-positive inflation caused by double dipping
ZOOM ID: 997 8258 4700 (Biomedical Mathematics Online Colloquium) (pw: 1234)Abstract: In typical single-cell RNA-seq (scRNA-seq) data analysis, a clustering algorithm is applied to find discrete cell clusters as putative cell types, and then a statistical test is employed to identify the differentially expressed (DE) genes between the cell clusters. However, this common procedure suffers the ``double dipping'' issue: the same data are used twice …