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TENET+: a tool for reconstructing gene networks by integrating single cell expression and chromatin accessibility data

July 5 @ 4:00 pm - 5:00 pm KST

B378 Seminar room, IBS, 55 Expo-ro Yuseong-gu
Daejeon, 34126 Korea, Republic of

Speaker

Junil Kim
Soongsil University

Reconstruction of gene regulatory networks (GRNs) is a powerful approach to capture a prioritized gene set controlling cellular processes. In our previous study, we developed TENET a GRN reconstructor from single cell RNA sequencing (scRNAseq). TENET has a superior capability to identify key regulators compared with other algorithms. However, accurate inference of gene regulation is still challenging. Here, we suggest an integrative strategy called TENET+ by combining single cell transcriptome and chromatin accessibility data. By applying TENET+ to a paired scRNAseq and scATACseq dataset of human peripheral blood mononuclear cells, we found critical regulators and their epigenetic regulations for the differentiations of CD4 T cells, CD8 T cells, B cells and monocytes. Interestingly, TENET+ predicted LRRFIP1 and ZBTB16 as top regulators of CD4 and CD8 T cells which were not predicted in a motif-based tool SCENIC. In sum, TENET+ is a tool predicting epigenetic gene regulatory programs in unbiased way, suggesting that novel epigenetic regulations can be identified by TENET+.

Details

Date:
July 5
Time:
4:00 pm - 5:00 pm KST
Event Category:

Venue

B378 Seminar room, IBS
55 Expo-ro Yuseong-gu
Daejeon, 34126 Korea, Republic of

Organizer

Jae Kyoung Kim
Email:
jaekkim@kaist.ac.kr
IBS 의생명수학그룹 Biomedical Mathematics Group
기초과학연구원 수리및계산과학연구단 의생명수학그룹
대전 유성구 엑스포로 55 (우) 34126
IBS Biomedical Mathematics Group (BIMAG)
Institute for Basic Science (IBS)
55 Expo-ro Yuseong-gu Daejeon 34126 South Korea
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