Cell signaling in 2D vs. 3D

Abstract:

The activation of Ras depends upon the translocation of its guanine nucleotide exchange factor, Sos, to the plasma membrane. Moreover, artificially inducing Sos to translocate to the plasma membrane is sufficient to bring about Ras activation and activation of Ras’s targets. There are many other examples of signaling proteins that must translocate to the membrane in order to relay a signal.

One attractive idea is that translocation promotes signaling by bringing a protein closer to its target. However, proteins that are anchored to the membrane diffuse more slowly than cytosolic proteins do, and it is not clear whether the concentration effect or the diffusion effect would be expected to dominate. Here we have used a reconstituted, controllable system to measure the association rate for the same binding reaction in 3D vs. 2D to see whether association is promoted, and, if so, how.