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New Frontier in Glioblastoma Theragnosis - Visualizing Tumor Microenvironment 게시판 상세보기
Title New Frontier in Glioblastoma Theragnosis - Visualizing Tumor Microenvironment
Embargo date 2024-01-16 00:00 Hits 290
Press release  
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New Frontier in Glioblastoma Theragnosis - Visualizing Tumor Microenvironment

- PET imaging of tumor microenvironment reveals new insights into glioblastoma pathology, offering a potential breakthrough in diagnosis and treatment -

Release Summary Text (75 words maximum)

A research team led by Prof. YUN Mijin at the department of Nuclear Medicine within the Severance Hospital along with Prof. CHANG Jong Hee, Prof. KANG Seok-Gu at the department of Neurosurgery within the Severance Hospital and Dr. C. Justin LEE at the Center for Cognition and Sociality (CCS) within the Institute for Basic Science (IBS) in Daejeon have developed a technology to visualize reactive astrocyte in the glioblastoma tumor microenvironment and have its mechanism.

Full text of release:

Recently, a team of South Korean researchers led by nuclear medicine physician and Professor YUN Mijin from Severance Hospital in collaboration with Director C. Justin LEE of the Center for Cognition and Sociality within the Institute for Basic Science (IBS) made a new discovery that can revolutionize both the diagnosis and treatment of glioblastoma. The group demonstrated a mechanism where the astrocytes in the brain uptake elevated levels of acetates, which turns them into hazardous reactive astrocytes. They then went on further to develop a new imaging technique that takes advantage of this mechanism to directly observe the tumor microenvironment.

Glioblastoma multiforme (GBM) is the most aggressive type of brain cancer with a median overall survival (OS) of approximately 15 months, and it accounts for the majority of primary malignant brain tumors. One of the hallmark features of GBM is the formation of a tumor microenvironment (TME). The TME comprises various cell types, including cancer stem cells and non-tumorous cells, such as astrocytes.

In TME, astrocytes undergo reactive astrogliosis which contributes to the proliferation, invasion, and drug resistance of glioblastoma. In general, glioblastomas are typically treated by surgically excising the tumor through total resection, and magnetic resonance imaging (MRI) is primarily utilized for preoperative localization.

These reactive astrocytes are known to excessively metabolize acetate, which allows them to be easily tracked via radioisotope imaging. To achieve this purpose, Yun and Lee’s research team introduced the use of Carbon-11 acetate (11C-acetate) as a probe for Positron Emission Tomography (PET). Earlier this year, they presented a study using 11C-acetate PET imaging to visualize reactive astrocytes in Alzheimer's patients. This time, Yun’s team ventured to apply the same approach to glioblastoma.

This approach was found to be particularly useful when tracking glioblastoma compared to other cancers. The 11C-acetate radiotracer is conventionally used to image cells that excessively uptake acetate, such as tumor cells. In the case of glioblastoma, both the reactive astrocytes in the TME uptake acetate as well as the tumor itself, which allows the PET imaging to track and distinguish both components of the cancer. (Figures 1A to 1D).

Nuclear medicine physician KIM Dongwoo, one of the first authors of this paper, stated, “PET imaging with 11C-acetate PET can prove to be an effective technique in diagnosing and determining the surgical resection areas of glioblastoma, showcasing significant clinical value.”

The research team looked further to see whether they could take advantage of the acetate metabolism feature for therapeutic purposes as well. Glioma cells are known to monopolize glucose for themselves, which leaves the tumor environment without much glucose, except for the glioma cells. The TME components such as reactive astrocytes are then forced to utilize acetate from the glioma cells for energy.

The astrocytes uptake acetate through a doorway called monocarboxylate transporter 1 (MCT1) (Figure 1E). When astrocytes take in too much acetate, it leads to a condition called reactive astrocytosis (see Figure 1F to 1G). In experiments with the mouse glioblastoma model, inhibiting reactive astrocytosis or blocking the expression of MCT1 was shown to restore acetate uptake in the tumor microenvironment to normal levels (Figure 2A to 2D).

Dr. YUN Mijin commented, “We showed that reactive astrocytes could be a great therapeutic target for glioblastoma,” and added, “Combining therapeutics and PET imaging with 11C-acetate as the radiotracer has significant importance in the aspect of 'Theragnosis (Therapy + Diagnosis)’.”

In addition, Yun and Lee’s team discovered that the prognosis of glioblastoma patients is less favorable when the areas of the TME identified through 11C-acetate PET imaging are larger compared to the tumor areas identified through MRI. This finding underscores the crucial role of the TME in the prognosis of glioblastoma patients.

The researchers emphasized the importance of the TME components, consisting of reactive astrocytes and cancer stem cells, in the progression and metastasis of tumors. They highlighted that successfully removing these components through surgical procedures is essential for the successful resection of tumors (Figures 3A to 3E).

Director C. Justin LEE stated, “Beyond tumor cells and surrounding reactive astrocytes, there is a need for subsequent macroscopic studies to explore how cancer stem cells, which constitute the TME, and the surrounding neurons influence each other.”




Figure 1. Regions of 11C-acetate uptake at the tumor boundary in patients with glioblastomas
Figure 1. Regions of 11C-acetate uptake at the tumor boundary in patients with glioblastomas
A. Representative images of 11C-acetate PET, and MRI in patients with glioblastoma and IDH1-mt astrocytoma.
B. SUVR of 11C-acetate uptake correlated with the WHO 2021 classification
C. The median tumor volume in 11C-acetate PET images was significantly larger than that seen on MRI in glioblastoma.
D. Western blot for MCT1 expression in mouse primary astrocytes and various human glioma cell lines (U87MG, U373MG, T98G, and U87mt).
E. 14C-acetate uptake in mouse primary astrocytes and various human glioma cell lines.
F. Immunofluorescence images showing GFAP, Ki-67, MAO-B, and MCT1 in glioblastoma patients.
G. Immunofluorescence images showing GFAP, Ki-67, MAO-B, and MCT1 in IDH1-mt astrocytoma.

Figure 2. 11C-acetate uptake is blocked by MAO-B inhibitor and MCT1 gene silencing in the patient-derived glioblastoma mice model (PDX mice model)
Figure 2. 11C-acetate uptake is blocked by MAO-B inhibitor and MCT1 gene silencing in the patient-derived glioblastoma mice model (PDX mice model)
A. Representative images of MRI, 11C-acetate PET, PET, and MRI fusion with or without KDS2010 administration in the PDX mice model.
B. SUVr of 11C-acetate in the peritumoral (Peri-T.) and contralateral (Ctr.) regions in Figure 2A.
C. Representative images of MRI, 11C-acetate PET, PET, and MRI fusion with or without astrocyte-specific MCT1 gene-silencing around PDX glioblastoma cells.
D. SUVr of 11C-acetate in the peritumoral (Peri-T.) and contralateral (Ctr.) regions in Figure 2C.

Figure 3. Relationship between volume of reactive astrogliosis and prognosis in patients with glioblastoma and cancer stem cells
Figure 3. Relationship between volume of reactive astrogliosis and prognosis in patients with glioblastoma and cancer stem cells
A. Immunofluorescence images showing GFAP, Ki-67, and CD133 in the peri-tumoral region of glioblastoma patients.
B. The proportion of GFAP+ and/or CD133+ in Ki-67+ cells in glioblastoma patients.
C. The 11C-acetate uptake area beyond the contrast-enhanced MRI was defined as VolumeACE-MRI.
D. Kaplan–Meier Survival analysis showed significant differences in progression-free survival (PFS) between the two groups with a VolumeACE-MRI cutoff value of > 5.06cc.
E. Kaplan–Meier Survival analysis also showed significant differences in overall survival (OS) between the two groups with a VolumesmallACE-MRI cutoff value of > 5.06cc.

Notes for editors

- References
Dongwoo Kim, Hae Young Young Ko, Jee-In Chung, Yongmin Mason Mason Park, Sangwon Lee, Seon Yoo Kim, Jisu Kim, Joong-Hyun Chun, Kyung-Seok Han, Misu Lee, Yeon Ha Ju, Sun Jun Park, Ki Duk Park, Min-Ho Nam, Se Hoon Kim, Jin-Kyoung Shim, Youngjoo Park, Hyunkeong Lim, Jaekyung Park, Hyunjin Kim, Gwan-Ho Lee, Suhyun Kim, Uiyeol Park, Kwan-Ho Lee, Hoon Ryu, So Yun Lee, Sunghyouk Park, Seok-Gu Kang, Jong Hee Chang, C. Justin Lee, Mijin Yun. Visualizing Cancer-Originating Acetate Uptake Through MCT1 in Reactive Astrocytes in the Glioblastoma Tumor Microenvironment. Neuro-oncology. DOI: https://doi.org/10.1093/neuonc/noad243


- Media Contact
For further information or to request media assistance, please contact Dr. C. Justin Lee at the Center for Cognition and Sociality, Institute for Basic Science (IBS) (cjl@ibs.re.kr) or William I. Suh at the IBS Public Relations Team (willisuh@ibs.re.kr).


- About the Institute for Basic Science (IBS)
IBS was founded in 2011 by the government of the Republic of Korea with the sole purpose of driving forward the development of basic science in South Korea. IBS has 6 research institutes and 30 research centers as of January 2024. There are eight physics, three mathematics, five chemistry, seven life science, two earth science, and five interdisciplinary research centers.


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