[특허 요약] The present invention successfully introduced a new approach to target specific EV subpopulations on the basis of charge-mediated fusion of EVs and CLIPs. By adjusting the surface charge of liposomes through the ratio of positively and negatively charged lipids, the optimal ratio that allows efficient and stable fusion with exosomes was confirmed. A method according to the present invention uses the advantages of a CLIP's high fusion rate, and rapid and broad applicability, and verified excellent sensitivity and selectivity for disease-derived EV miRNA in a lysis-free manner using droplet-microfluidics. Particularly, the EV-CLIP method enables digital detection of EGFR L858R and T790M mutations without pretreating a sample, and thus can simplify detection processes and prevent EV loss.

등록번호

20250369057A

출원번호

202519235274

출원인

기초과학연구원, 울산과학기술원

키워드

dimethyl, cancer, liposome, disease, bis