Cytosine base-editing composition and use of same

[특허 요약] The present invention relates to a cytosine base-editing composition, and more specifically, to a cytosine base-editing composition comprising adenine deaminase and CRISPR-associated protein 9 (Cas9), or functional analogs thereof, which has a narrow editing window and thus is capable of accurately editing cytosine in a target sequence with another base.The present inventors have discovered that the application of the cytosine base-editing composition comprising adenine deaminase and Cas9, or functional analogs thereof, to a target sequence, causes cytosine on a specific motif to be substituted with another base, and that existing adenine base editors (ABEs) are capable of editing not only adenine but also cytosine, and can also have said substitutions occur with higher accuracy compared to existing cytosine base editors (CBEs). Thus, the cytosine base-editing composition according to the present invention is expected to be advantageously used in the treatment of genetic disorders and in research on genetic disorders.

특허 등록번호

20220315915A

특허 출원번호

202017630774

출원일

2020.07.29

등록일

2022.10.06

국가

미국

출원인

한양대학교 산학협력단, 기초과학연구원

발명자

Sangsu BAE, Heon Seok KIM, You Kyeong JEONG

중요 키워드

cytosine, editing, base, present, adenine