Method of identifying genome-wide off-target sites of base editors by detecting single strand breaks in genomic dna

[특허 요약] Provided are a composition for inducing DNA single strand breaks in DNA, the composition comprising a cytidine deaminase, an inactivated target-specific endonuclease, and a guide RNA, a method for inducing a single-strand break in DNA, using the same, a method for analyzing a nucleic acid sequence of a base-editing-introduced DNA, and a method for identifying (or measuring or detecting) a base-editing site, base-editing efficiency at an on-target site, an off-target site, and/or target specificity.

특허 등록번호

20180258418A

특허 출원번호

201815872907

출원일

2018.01.16

등록일

2018.09.13

국가

미국

출원인

기초과학연구원

발명자

Jin-soo Kim

중요 키워드

emx1, dna, target, site, strand