March 16, 2016

Lactobacillus might sound familiar when the topic of probiotics comes up, but they are only one of many types of bacteria that have proven health benefits. In a new study by the Academy for Immunology and Microbiology within the Institute for Basic Science (IBS) and the National Institute of Animal Science research project of Rural Development Administration, researchers have shown that the introduction of the probiotic Bifidobacterium longum KACC 91563 has the ability to reduce the effects of food allergies. In the US, the number of children with food allergies increased by 18% between 1997 and 2007; during the same time allergy-related hospital visits tripled ¹

Though some probiotics have proven to be very effective for reducing food allergies, this is not the case for all probiotic bacteria. The IBS team tested two strains of bacteria, Bifidobacterium longum KACC 91563 and Enterococcus faecalis KACC 91532, on mice which were later exposed to food allergens to see what effect they had on immune system response. Mice with an E. faecalis KACC 91532 intestinal population did not show any change in allergic response, however in mice with B. longum KACC 91563 intestinal populations there was a significant suppression and delay in the occurrence of diarrhea.

▲ Picture of B. Longum KACC 91563

The probiotics work by releasing extracellular vesicles (EVs), tiny spherical packets that contain protein and DNA which are released into the small and large intestines. The EVs deliver this material from the bacteria which influence the immune system. In the case of B. longum KACC 91563, the EVs contain family 5 extracellular solute-binding protein (ESBP). In the intestines, the ESBP interacts with mast cells which are responsible for allergic response, and causes them to kill themselves, thus neutralizing their ability to induce allergy.

The mice were given a common test allergen, albumin, the protein found in egg whites, mixed with alum to induce food allergy responses. The team looked for signs of intestinal distress, in this case diarrhea, as an indicator of an allergic response. They found that the dose was an important factor, as less than 5 x 109 colony forming units (cfu) of bacteria per mouse per day were not enough to prevent allergic response from occurring.

▲ EVs interacting with mast cells causing apoptosis

While the number of food allergies cases continues to grow, few treatment options exist. Dr. Yang stressed, “Our study is the first to discover the probiotic strain’s mechanism of controlling food allergies without affecting regulatory T cells” and added, “since mast cells are the root cause of all allergic reactions, recombinant ESBP protein might be used therapeutic treatment of other allergic diseases as well as food allergy”. There are other possible applications for ESBP including in skin cream to treat eczema. This protein treatment looks like a significant step in mitigating an overactive immune response which will go a long way in combating allergies.

The study was published in February by the Journal of Allergy and Clinical Immunology.

¹ Branum AM, Lukacs SL. Food allergy among children in the United States. Pediatrics 2009;124:1549-55.

Daniel Kopperud

Notes for editors

- References
Jung-Hwan Kim, PhD, Eun-Ji Jeun, MSc, Chun-Pyo Hong, PhD, Seong-Hoon Kim, MSc, Min Seong Jang, DVM, PhD, Eun-Jung Lee, MSc, Sook Jin Moon, BSc, Chang Ho Yun, PhD, Sin-Hyeog Im, PhD, Seok-Geun Jeong, PhD, Beom-Young Park, PhD, Kyong-Tai Kim, PhD, Ju-Young Seoh, MD, PhD, Yoon-Keun Kim, MD, PhD, Sung-Jong Oh, PhD, Jun-Sang Ham, PhD, Bo-Gie Yang, PhD, and Myoung Ho Jang, PhD; Extracellular vesicle–derived protein from Bifidobacterium longum alleviates food allergy through mast cell suppression; American Academy of Allergy, Asthma & Immunology, DOI: http://dx.doi.org/10.1016/j.jaci.2015.08.016

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- About the Institute for Basic Science (IBS)
IBS was founded in 2011 by the government of the Republic of Korea with the sole purpose of driving forward the development of basic science in Korea It comprises a total of 50 research centers in all fields of basic science, including mathematics, physics, chemistry, life science, earth science and interdisciplinary science. IBS has launched 26 research centers as of March 2016. There are 8 physics, 1 mathematics, 6 chemistry, 8 life science, and 3 interdisciplinary research centers.