This talk will be given online.<\/p>\n
Abstract:<\/p>\n
Synthetic biology and microbial biotechnology offer sustainable routes to the manufacture of commodity and high value chemicals from agricultural by-products instead of petrochemical feedstocks. However, engineered gene circuits and metabolic pathways both co-opt the cell\u2019s gene expression machinery for protein\/enzyme production and divert metabolic flux away from key host biosynthetic building blocks to a desired product. These interactions impair host growth and complicate the engineering of synthetic functions. To overcome these difficulties, we propose a host-aware engineering approach which accounts for these constraints during the circuit\/pathway design process. Here we present a dynamic whole cell modelling framework of microbial growth, metabolism, and gene expression which captures key host-circuit\/pathway interactions. By coupling our modelling framework with systems engineering approaches and multi-objective optimization tools, we identify key design trade-offs, make recommendations for optimal host resource usage, and develop feedback control strategies which improve pathway productivity and yields.<\/p>\n","protected":false},"excerpt":{"rendered":"
This talk will be given online. Abstract: Synthetic biology and microbial biotechnology offer sustainable routes to the manufacture of commodity and high value chemicals from agricultural by-products instead of petrochemical … <\/p>\n