{"id":4304,"date":"2021-03-23T19:50:30","date_gmt":"2021-03-23T10:50:30","guid":{"rendered":"https:\/\/www.ibs.re.kr\/bimag\/?post_type=tribe_events&#038;p=4304"},"modified":"2021-04-07T13:10:48","modified_gmt":"2021-04-07T04:10:48","slug":"2021-04-09","status":"publish","type":"tribe_events","link":"https:\/\/www.ibs.re.kr\/bimag\/event\/2021-04-09\/","title":{"rendered":"Highly accurate fluorogenic DNA sequencing with information theory\u2013based error correction"},"content":{"rendered":"<p>We will discuss about &#8220;Highly accurate fluorogenic DNA sequencing with information theory\u2013based error correction&#8221;, Chen <em>et al.<\/em>, Nature Biotechnology (2017)<\/p>\n<p>Eliminating errors in next-generation DNA sequencing has proved challenging. Here we present error-correction code (ECC) sequencing, a method to greatly improve sequencing accuracy by combining fluorogenic sequencing-by-synthesis (SBS) with an information theory\u2013based error-correction algorithm. ECC embeds redundancy in sequencing reads by creating three orthogonal degenerate sequences, generated by alternate dual-base reactions. This is similar to encoding and decoding strategies that have proved effective in detecting and correcting errors in information communication and storage. We show that, when combined with a fluorogenic SBS chemistry with raw accuracy of 98.1%, ECC sequencing provides single-end, error-free sequences up to 200 bp. ECC approaches should enable accurate identification of extremely rare genomic variations in various applications in biology and medicine.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>We will discuss about &#8220;Highly accurate fluorogenic DNA sequencing with information theory\u2013based error correction&#8221;, Chen et al., Nature Biotechnology (2017) Eliminating errors in next-generation DNA sequencing has proved challenging. Here &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/www.ibs.re.kr\/bimag\/event\/2021-04-09\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Highly accurate fluorogenic DNA sequencing with information theory\u2013based error correction&#8221;<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"template":"","meta":{"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}","_uag_custom_page_level_css":"","_tribe_events_status":"","_tribe_events_status_reason":"","footnotes":""},"tags":[260,259,258],"tribe_events_cat":[219],"class_list":["post-4304","tribe_events","type-tribe_events","status-publish","hentry","tag-information-theory","tag-next-generation-sequencing","tag-ngs","tribe_events_cat-journal-club","cat_journal-club"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Highly accurate fluorogenic DNA sequencing with information theory\u2013based error correction - Biomedical Mathematics Group<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.ibs.re.kr\/bimag\/event\/2021-04-09\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Highly accurate fluorogenic DNA sequencing with information theory\u2013based error correction - Biomedical Mathematics Group\" \/>\n<meta property=\"og:description\" content=\"We will discuss about &#8220;Highly accurate fluorogenic DNA sequencing with information theory\u2013based error correction&#8221;, Chen et al., Nature Biotechnology (2017) Eliminating errors in next-generation DNA sequencing has proved challenging. 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Here &hellip; Continue reading \"Highly accurate fluorogenic DNA sequencing with information theory\u2013based error correction\"","og_url":"https:\/\/www.ibs.re.kr\/bimag\/event\/2021-04-09\/","og_site_name":"Biomedical Mathematics Group","article_modified_time":"2021-04-07T04:10:48+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.ibs.re.kr\/bimag\/event\/2021-04-09\/","url":"https:\/\/www.ibs.re.kr\/bimag\/event\/2021-04-09\/","name":"Highly accurate fluorogenic DNA sequencing with information theory\u2013based error correction - Biomedical Mathematics Group","isPartOf":{"@id":"https:\/\/www.ibs.re.kr\/bimag\/#website"},"datePublished":"2021-03-23T10:50:30+00:00","dateModified":"2021-04-07T04:10:48+00:00","breadcrumb":{"@id":"https:\/\/www.ibs.re.kr\/bimag\/event\/2021-04-09\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.ibs.re.kr\/bimag\/event\/2021-04-09\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.ibs.re.kr\/bimag\/event\/2021-04-09\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.ibs.re.kr\/bimag\/"},{"@type":"ListItem","position":2,"name":"Events","item":"https:\/\/www.ibs.re.kr\/bimag\/events\/"},{"@type":"ListItem","position":3,"name":"Highly accurate fluorogenic DNA sequencing with information theory\u2013based error correction"}]},{"@type":"WebSite","@id":"https:\/\/www.ibs.re.kr\/bimag\/#website","url":"https:\/\/www.ibs.re.kr\/bimag\/","name":"Biomedical Mathematics Group","description":"\uae30\ucd08\uacfc\ud559\uc5f0\uad6c\uc6d0 \uc758\uc0dd\uba85\uc218\ud559\uadf8\ub8f9","publisher":{"@id":"https:\/\/www.ibs.re.kr\/bimag\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.ibs.re.kr\/bimag\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.ibs.re.kr\/bimag\/#organization","name":"IBS Biomedical Mathematics Group","url":"https:\/\/www.ibs.re.kr\/bimag\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.ibs.re.kr\/bimag\/#\/schema\/logo\/image\/","url":"https:\/\/www.ibs.re.kr\/bimag\/cms\/wp-content\/uploads\/2021\/02\/ibs-circle-1.png","contentUrl":"https:\/\/www.ibs.re.kr\/bimag\/cms\/wp-content\/uploads\/2021\/02\/ibs-circle-1.png","width":250,"height":250,"caption":"IBS Biomedical Mathematics Group"},"image":{"@id":"https:\/\/www.ibs.re.kr\/bimag\/#\/schema\/logo\/image\/"}}]}},"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"dimag-thumbnail":false,"twentyseventeen-featured-image":false,"twentyseventeen-thumbnail-avatar":false},"uagb_author_info":{"display_name":"BIMAG","author_link":"https:\/\/www.ibs.re.kr\/bimag\/author\/hphongblog\/"},"uagb_comment_info":0,"uagb_excerpt":"We will discuss about &#8220;Highly accurate fluorogenic DNA sequencing with information theory\u2013based error correction&#8221;, Chen et al., Nature Biotechnology (2017) Eliminating errors in next-generation DNA sequencing has proved challenging. Here &hellip; Continue reading \"Highly accurate fluorogenic DNA sequencing with information theory\u2013based error correction\"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.ibs.re.kr\/bimag\/wp-json\/wp\/v2\/tribe_events\/4304","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ibs.re.kr\/bimag\/wp-json\/wp\/v2\/tribe_events"}],"about":[{"href":"https:\/\/www.ibs.re.kr\/bimag\/wp-json\/wp\/v2\/types\/tribe_events"}],"author":[{"embeddable":true,"href":"https:\/\/www.ibs.re.kr\/bimag\/wp-json\/wp\/v2\/users\/3"}],"version-history":[{"count":2,"href":"https:\/\/www.ibs.re.kr\/bimag\/wp-json\/wp\/v2\/tribe_events\/4304\/revisions"}],"predecessor-version":[{"id":4392,"href":"https:\/\/www.ibs.re.kr\/bimag\/wp-json\/wp\/v2\/tribe_events\/4304\/revisions\/4392"}],"wp:attachment":[{"href":"https:\/\/www.ibs.re.kr\/bimag\/wp-json\/wp\/v2\/media?parent=4304"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ibs.re.kr\/bimag\/wp-json\/wp\/v2\/tags?post=4304"},{"taxonomy":"tribe_events_cat","embeddable":true,"href":"https:\/\/www.ibs.re.kr\/bimag\/wp-json\/wp\/v2\/tribe_events_cat?post=4304"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}